Tryptophan Insertion Mutagenesis of Liver Fatty Acid-binding Protein
نویسندگان
چکیده
منابع مشابه
Sex steroid modulation of fatty acid utilization and fatty acid binding protein concentration in rat liver.
The mechanism by which sex steroids influence very low density hepatic lipoprotein triglyceride production has not been fully elucidated. In previous studies we showed that [(14)C]oleate utilization and incorporation into triglycerides were greater in hepatocyte suspensions from adult female rats than from males. The sex differences were not related to activities of the enzymes of triglyceride ...
متن کاملStudies on fatty-acid-binding proteins. The purification of rat liver fatty-acid-binding protein and the role of cysteine-69 in fatty acid binding.
1. A new, simple and high-yield procedure is described for the purification of hepatic fatty-acid-binding protein from rat liver using naphthylaminodecyl-agarose as an affinity column. 2. Cysteine-69 is shown to react slowly, but quantitatively, with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB), indicating that the thiol group is free, but may be buried within the protein. 3. Fatty acids do not ...
متن کاملEnterocyte fatty acid uptake and intestinal fatty acid-binding protein.
This article reviews our current understanding of the uptake of fatty acids by the enterocytes of the intestine. The micellar solubilization of fatty acids by bile salts and the factors regulating that process are discussed. The mechanism of how micellar solubilization of fatty acids promotes the uptake of fatty acids by enterocytes and their relative importance is reviewed. Additionally, discu...
متن کاملEffects of feed restriction and dietary fat type on mRNA expression of liver fatty acid-binding protein (L-FABP) in broilers
Background: Liver fatty acid-binding protein (L-FABP) is the main cytosolic binding site for long chain fatty acids in hepatocytes. FABPs enhance the uptake of fatty acids into the cell by increasing their concentration due to decreasing concentration of unbound fatty acids inside the cell. Objectives: The aim of this study was to evaluate the effects of dietary unsaturated to saturated fatty a...
متن کاملFatty acid-binding protein from embryonic chick retina resembles mammalian heart fatty acid-binding protein.
PURPOSE Fatty acid-binding proteins (FABP) make up a family of cytosolic proteins of unknown function found in many tissues, including the embryonic chick retina. To further understand its function in the developing retina, the protein from embryonic chicks was purified and characterized. The tissue distribution of this protein in embryonic and hatched chicks was determined using polyclonal ant...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2005
ISSN: 0021-9258
DOI: 10.1074/jbc.m407131200